Using Atomic Force Microscopy to Measure Mechanical Strength of Nanometre Scale Protein Fibrils
نویسندگان
چکیده
منابع مشابه
Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
Matrix stiffness strongly influences growth, differentiation and function of adherent cells. On the macro scale the stiffness of tissues and organs within the human body span several orders of magnitude. Much less is known about how stiffness varies spatially within tissues, and what the scope and spatial scale of stiffness changes are in disease processes that result in tissue remodeling. To b...
متن کاملMechanical Characterisation of HeLa Cells using Atomic Force Microscopy
Recently, atomic force microscopy (AFM) has been shown to be a suitable tool for imaging biological structures and their modification, adding to accurate morphological and cytomechanical information. AFM is capable of simultaneous nanometer spatial resolution and piconewton force detection, allowing detailed study of a cell surface morphology and monitoring of cell-tip interactions. Modern AFM ...
متن کاملAtomic-Scale Variations of the Mechanical Response of 2D Materials Detected by Noncontact Atomic Force Microscopy.
We show that noncontact atomic force microscopy (AFM) is sensitive to the local stiffness in the atomic-scale limit on weakly coupled 2D materials, as graphene on metals. Our large amplitude AFM topography and dissipation images under ultrahigh vacuum and low temperature resolve the atomic and moiré patterns in graphene on Pt(111), despite its extremely low geometric corrugation. The imaging me...
متن کاملCharacterization of Protein–Protein Interactions Using Atomic Force Microscopy
Atomic force microscopy (AFM), invented in 1986, expanded the application of scanning tunneling microscopy to nonconductive, soft, and live biological samples (Binnig et al., 1986; Hansma et al., 1988; Marti et al., 1988; Drake et al., 1989). AFM has several capabilities, including characterizing topographic details of surfaces from the submolecular level to the cellular level (Radmacher et al....
متن کاملInvestigation of protein-protein interaction using atomic force microscopy
INVESTIGATION OF PROTEIN-PROTEIN INTERACTION USINGATOMIC FORCE MICROSCOPYbyEssa Y. MayyasAugust 2011Advisor: Dr. Peter M. HoffmannMajor: PhysicsDegree: Doctor of Philosophy Single-molecule approaches to measuring the kinetics of protein dissociation have theadvantage that measurements can be performed in native environments, such as living cells,where concent...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.2021